Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering.
نویسندگان
چکیده
Time-resolved small-angle x-ray scattering was used to measure the radius of gyration of cytochrome c after initiation of folding by a pH jump. Submillisecond time resolution was obtained with a microfabricated diffusional mixer and synchrotron radiation. The results show that the protein first collapses to compact denatured structures before folding very fast to the native state.
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 96 18 شماره
صفحات -
تاریخ انتشار 1999